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Rapid and Simple GC–MS Method for Determination of Psychotropic Phenylalkylamine Derivatives in Nails Using Micro-Pulverized Extraction

Authors


  • Partially funded by the National R&D Program of Ministry of Education, Science and Technology (MEST) and National Research Foundation (NRF) of Korea.

Additional information and reprint requests:
Jin Young Kim, Ph.D.
Drug Analysis Laboratory
Supreme Prosecutors’ Office
706 Banporo, Seochogu
Seoul 137-730
Korea
E-mail: paxus@spo.go.kr

Abstract

Abstract:  A rapid and simple gas chromatography–mass spectrometry (GC–MS) method was developed and validated for the simultaneous detection and quantification of five psychotropic phenylalkylamines (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and norketamine) in toenails. After external decontamination, nail clippings were mechanically pulverized with a bead mill and then incubated in methanol under ultrasonication at 50°C for 1 h. The resulting solutions were evaporated to dryness, derivatized, and analyzed by GC–MS. The intra- and inter-day precisions were within 10.7% and 13.9%, respectively. The intra- and inter-day accuracies were −4.2% to 5.0% and −2.4% to 8.4%, respectively. Limits of detection and quantification for each analyte were lower than 0.024 and 0.08 ng/mg, respectively. The recoveries were in the range of 80.6–87.5%. The results indicated that the proposed method is a simple, rapid, accurate, and precise for quantification of five phenylalkylamines in nails. The method was successfully applied to the simultaneous detection and quantification of phenylalkylamines in nail samples of possible drug abusers.

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