Supported in part by a grant from IFIMAV (API 11/26).
TECHNICAL NOTE CRIMINALISTICS
Haplotypic Blocks of X-linked STRs for Forensic Cases: Study of Recombination and Mutation Rates*
Article first published online: 16 SEP 2011
© 2011 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 1, pages 192–195, January 2012
How to Cite
Castañeda, M., Mijares, V., Riancho, J. A. and Zarrabeitia, M. T. (2012), Haplotypic Blocks of X-linked STRs for Forensic Cases: Study of Recombination and Mutation Rates. Journal of Forensic Sciences, 57: 192–195. doi: 10.1111/j.1556-4029.2011.01916.x
- Issue published online: 4 JAN 2012
- Article first published online: 16 SEP 2011
- Received 30 May 2010; and in revised form 24 Nov. 2010; accepted 26 Nov. 2010.
- forensic science;
- DNA typing;
- short tandem repeat;
Abstract: In complex kinship cases, markers situated in haplotypic blocks may provide additional clues to other unlinked markers. We have established a protocol to amplify six X-chromosome microsatellites, located in two haplotype blocks, using PCR with fluorochrome-labeled primers and capillary electrophoresis. The segregation stability was explored in 92 unrelated families with individuals from three generations. Sixty-one different haplotypes were found in the DXS10079-DXS10074-DXS10075 block in the grandfathers and 96 in the mothers, with estimated haplotype diversities of 0.9828 and 0.9842, respectively. Fifty and 73 different haplotypes were found in the DXS6801-DXS6809-DXS6789 block in the grandfathers and the mothers, with estimated haplotype diversities of 0.9711 and 0.9742, respectively. We observed 10 between-cluster and one within-cluster recombinations in 99 female meioses. The overall per-locus mutation rate was 0.0034. This protocol allows for the characterization of the alleles of two sets of linked markers of the X-chromosome that can be useful in complex forensic cases.