Supported by the Parco Nazionale dei Monti Sibillini research project, “Fingerprint genetico miele.”
TECHNICAL NOTE PATHOLOGY/BIOLOGY; TOXICOLOGY
Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens*
Article first published online: 10 NOV 2011
© 2011 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 1, pages 222–227, January 2012
How to Cite
Olivieri, C., Marota, I., Rollo, F. and Luciani, S. (2012), Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens. Journal of Forensic Sciences, 57: 222–227. doi: 10.1111/j.1556-4029.2011.01964.x
- Issue published online: 4 JAN 2012
- Article first published online: 10 NOV 2011
- Received 16 April 2010; and in revised form 1 Dec. 2010; accepted 12 Dec. 2010.
- forensic science;
- honey DNA;
- bee symbionts;
Abstract: Consuming honey can result in adverse effects owing to poisoning by bacterial (botulism) or plant toxins. We have devised a method to extract polymerase chain reaction (PCR) amplifiable DNA of up to c. 400 bp in length based on dialysis of a 15-mL honey sample for 18 h against deionized water followed by sequential extraction using phenol, phenol/chloroform/isoamyl alcohol, chloroform/isoamyl alcohol, and ether. Sequence analysis of PCR products obtained using “universal” plant, fungal, and bacterial primers targeted to the ribosomal RNA genes has allowed us to identify six different orders of plants (Apiales, Fabales, Asterales, Solanales, Brassicales, and Sapindales), two orders of fungi (Entylomatales and Saccharomycetales), and six orders of bacteria (Sphingomonadales, Burkholderiales, Pseudomonadales, Enterobacteriales, Actinomycetales, and Bifidobacteriales) in a single honey specimen.