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Comparison of Two Methods for Isolating DNA from Human Skeletal Remains for STR Analysis

Authors

  • Cynthia Rucinski M.D.,

    1. Grupo de identificación Humana e Inmunogenética, Universidad Nacional de Colombia, Bogotá, D.C., Colombia.
    2. Departamento de Patología, Facultad de Medicina e Instituto de Genética, Universidad Nacional de Colombia, Kra 30 # 45-03, Bogotá, D.C., Colombia.
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  • Ayda L. Malaver B.S.,

    1. Instituto de Genética, Servicios Médicos Yunis Turbay y Cia. Kra 24 · 42-24, Bogotá, D.C., Colombia.
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  • Emilio J. Yunis M.D.,

    1. Instituto de Genética, Servicios Médicos Yunis Turbay y Cia. Kra 24 · 42-24, Bogotá, D.C., Colombia.
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  • Juan J. Yunis M.D.

    1. Grupo de identificación Humana e Inmunogenética, Universidad Nacional de Colombia, Bogotá, D.C., Colombia.
    2. Departamento de Patología, Facultad de Medicina e Instituto de Genética, Universidad Nacional de Colombia, Kra 30 # 45-03, Bogotá, D.C., Colombia.
    3. Instituto de Genética, Servicios Médicos Yunis Turbay y Cia. Kra 24 · 42-24, Bogotá, D.C., Colombia.
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Additional information and reprint requests:
Juan J. Yunis, M.D.
Profesor Titular
Departamento de Patología
Facultad de Medicina
Universidad Nacional de Colombia
Kra 30 · 45-03, Edificio 426
Or Servicios Médicos Yunis Turbay y Cia.
Kra 24 # 42-24, Of 102
Bogotá, D.C. Sur América
Colombia
E-mail: jjyunisl@unal.edu.co and jjy@yunis.com.co

Abstract

Abstract:  The quality and efficiency of a standard organic DNA isolation method and a silica-based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real-time PCR, and STR profiles were obtained using the AmpFlSTR® Identifiler® PCR amplification kit. Overall, the silica-based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica-based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica-based method does not improve neither the quality nor the quantity of DNA for STR profiling.

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