Funded by the National Natural Science Foundation of China (No. 30821005, 20805031, and 21035004), the Scientific Technology Support Project (No. 2007 BAK26B06), the National Key Basic Research Program (973 Program) of China (No. 2009CB118906), and the Shanghai Leading Academic Discipline Project (B203).
TECHNICAL NOTE TOXICOLOGY
Sensitive Determination of Barbiturates in Biological Matrix by Capillary Electrophoresis Using Online Large-Volume Sample Stacking*
Article first published online: 6 JAN 2012
© 2012 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 3, pages 813–819, May 2012
How to Cite
Fan, L.-Y., He, T., Tang, Y.-Y., Zhang, W., Song, C.-J., Zhao, X., Zhao, X.-Y. and Cao, C.-X. (2012), Sensitive Determination of Barbiturates in Biological Matrix by Capillary Electrophoresis Using Online Large-Volume Sample Stacking. Journal of Forensic Sciences, 57: 813–819. doi: 10.1111/j.1556-4029.2011.02034.x
The first two authors have contributed equally to the paper.
- Issue published online: 19 APR 2012
- Article first published online: 6 JAN 2012
- Received 12 Oct. 2010; and in revised form 21 Jan. 2011; accepted 8 Apr. 2011.
- forensic science;
- capillary zone electrophoresis;
- sample stacking;
- large volume
Abstract: In China, some forensic cases are caused by barbiturates. Thus, the determination of trace level barbiturates in body fluid is important for the poisoning investigation. In this study, an online large-volume sample stacking (LVSS) with polarity switching in capillary electrophoresis (CE) was applied for the sensitive determination of barbiturates. This technique involves injecting a large volume of sample into a capillary and removing the sample matrix plug out of the capillary by reversing the polarity. Quantitation limit obtained was 0.048, 0.057, 0.039, and 0.015 μg/mL for secobarbital, amobarbital, barbital, and phenobarbital (signal-to-noise ratio = 9). By using LVSS, the stacking was simply achieved at 171.7-, 169.7-, 202.7-, and 169.1-fold for the above four barbiturates. The relative standard deviation values of intraday and interday were <2.11% and 4.69%, respectively. Recoveries were ranged from 83.7 to 105.2%. Finally, the trace analysis method was applied to the analysis of real forensic specimens and has achieved satisfactory results.