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Sensitive Determination of Barbiturates in Biological Matrix by Capillary Electrophoresis Using Online Large-Volume Sample Stacking

Authors

  • Liu-Yin Fan Ph.D.,

    1. Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
    2. State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
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  • Ting He M.E.,

    1. Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
    2. State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
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  • Yun-Yun Tang M.E.,

    1. Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
    2. State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
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  • Wei Zhang Ph.D.,

    1. Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
    2. State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
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  • Chao-Jin Song B.E.,

    1. Forensic Science Institute of Beijing, Beijing 100129, China.
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  • Xia Zhao M.E.,

    1. Forensic Science Institute of Beijing, Beijing 100129, China.
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  • Xiao-Yu Zhao M.E.,

    1. Public Security Bureau, Forensic Science Institute of Jilin Province, Changchun 130021, China.
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  • Cheng-Xi Cao Ph.D.

    1. Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
    2. State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, Shanghai 200240, China.
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  • Funded by the National Natural Science Foundation of China (No. 30821005, 20805031, and 21035004), the Scientific Technology Support Project (No. 2007 BAK26B06), the National Key Basic Research Program (973 Program) of China (No. 2009CB118906), and the Shanghai Leading Academic Discipline Project (B203).

  • The first two authors have contributed equally to the paper.

Additional information and reprint requests:
Cheng-Xi Cao, Ph.D.
School of Life Science and Biotechnology
Shanghai Jiao Tong University
800 Dongchuan Road, Minhang
Shanghai 200240
China
E-mail: cxcao@sjtu.edu.cn

Abstract

Abstract:  In China, some forensic cases are caused by barbiturates. Thus, the determination of trace level barbiturates in body fluid is important for the poisoning investigation. In this study, an online large-volume sample stacking (LVSS) with polarity switching in capillary electrophoresis (CE) was applied for the sensitive determination of barbiturates. This technique involves injecting a large volume of sample into a capillary and removing the sample matrix plug out of the capillary by reversing the polarity. Quantitation limit obtained was 0.048, 0.057, 0.039, and 0.015 μg/mL for secobarbital, amobarbital, barbital, and phenobarbital (signal-to-noise ratio = 9). By using LVSS, the stacking was simply achieved at 171.7-, 169.7-, 202.7-, and 169.1-fold for the above four barbiturates. The relative standard deviation values of intraday and interday were <2.11% and 4.69%, respectively. Recoveries were ranged from 83.7 to 105.2%. Finally, the trace analysis method was applied to the analysis of real forensic specimens and has achieved satisfactory results.

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