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A Population Genetic Database of Cat Breeds Developed in Coordination with a Domestic Cat STR Multiplex

Authors


  • Supported by an Interagency Grant (IAA # 1999-IJ-R-A079) awarded by the National Institute of Justice, Office of Justice Programs, US Department of Justice to the National Cancer Institute’s Laboratory of Genomic Diversity. BSW has been supported in part by NIH grant GM 75091. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the US Department of Justice.

Additional information and reprint requests:
Marilyn Menotti-Raymond, Ph.D.
NCI-Frederick
Building 560, Room 11-38
Frederick, MD 21702-1201
E-mail: raymondm@mail.nih.gov

Abstract

Abstract:  A simple tandem repeat (STR) PCR-based typing system developed for the genetic individualization of domestic cat samples has been used to generate a population genetic database of domestic cat breeds. A panel of 10 tetranucleotide STR loci and a gender-identifying sequence tagged site (STS) were co-amplified in genomic DNA of 1043 individuals representing 38 cat breeds. The STR panel exhibits relatively high heterozygosity in cat breeds, with an average 10-locus heterozygosity of 0.71, which represents an average of 38 breed-specific heterozygosities for the 10-member panel. When the entire set of breed individuals was analyzed as a single population, a heterozygosity of 0.87 was observed. Heterozygosities obtained for the 10 loci range from 0.72 to 0.96. The power for genetic individualization of domestic cat samples of the multiplex is high, with a probability of match (pm) of 6.2E-14, using a conservative θ = 0.05.

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