Funding received from the National Science Foundation (0745590).
TECHNICAL NOTE CRIMINALISTICS
Comparison of Bulk and Compound-Specific δ13C Isotope Ratio Analyses for the Discrimination Between Cannabis Samples*
Article first published online: 31 JAN 2012
© 2012 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 3, pages 757–764, May 2012
How to Cite
Muccio, Z., Wöckel, C., An, Y. and Jackson, G. P. (2012), Comparison of Bulk and Compound-Specific δ13C Isotope Ratio Analyses for the Discrimination Between Cannabis Samples. Journal of Forensic Sciences, 57: 757–764. doi: 10.1111/j.1556-4029.2011.02046.x
- Issue published online: 19 APR 2012
- Article first published online: 31 JAN 2012
- Received 24 Aug. 2010; and in revised form 17 Feb. 2011; accepted 30 April 2011.
- forensic science;
- isotope ratio mass spectrometry;
- compound-specific isotope ratios;
- bulk isotope ratios;
- gas chromatography;
- mass spectrometry;
- carbon isotope ratio;
- drugs of abuse
Abstract: Five marijuana samples were compared using bulk isotope analysis compound-specific isotope ratio analysis of the extracted cannabinoids. Owing to the age of our cannabis samples, four of the five samples were compared using the isotope ratios of cannabinol (CBN), a stable degradation product of Δ9-tetrahydrocannabinol (THC). Bulk δ13C isotope analysis discriminated between all five samples at the 95% confidence level. Compound-specific δ13C isotope analysis could not distinguish between one pair of the five samples at the 95% confidence level. All the measured cannabinoids showed significant depletion in 13C relative to bulk isotope values; the isotope ratios for THC, CBN, and cannabidiol were on average 1.6‰, 1.7‰, and 2.2‰ more negative than the bulk values, respectively. A more detailed investigation needs to be conducted to assess the degree fractionation between the different cannabinoids, especially after aging.