Supported by National Natural Science Foundation, PR China (No. 20975070) and National Institute scientific program (No. GY0903).
Hair as a Specimen to Document Tetramethylene Disulfotetramine Exposure*
Article first published online: 28 FEB 2012
© 2012 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 3, pages 669–673, May 2012
How to Cite
Shen, M., Xiang, P., Zhou, F., Shen, B. and Shi, Y. (2012), Hair as a Specimen to Document Tetramethylene Disulfotetramine Exposure. Journal of Forensic Sciences, 57: 669–673. doi: 10.1111/j.1556-4029.2012.02052.x
- Issue published online: 19 APR 2012
- Article first published online: 28 FEB 2012
- Received 29 June 2010; and in revised form 21 Feb. 2011; accepted 5 Mar. 2011.
- forensic science;
- forensic toxicology;
- tetramethylene disulfotetramine
Abstract: Tetramethylene disulfotetramine (tetramine) is a rodenticide that has been banned for many years in China. Since 2005, inhabitants of a village in the Henan Province have been suffering from grand mal seizures. To investigate the possibility of tetramine as the cause, we developed a method to determine tetramine in human hair. Sample preparation involved external decontamination, frozen pulverization, and ultrasonication in 2 mL ethyl acetate in the presence of cocaine-d3 as an internal standard. The method exhibited good linearity; calibration curve was linear over a range of 0.1–20 ng/mg hair. The limit of detection for the assay was 0.05 ng/mg hair. Except for one subject (No. 4), all head and pubic hair samples were positive for tetramine. The concentrations of tetramine in pubic hair were significantly higher than those in the same subjects’ head hair samples. Because of a long retention in body, segmental head hair analysis cannot provide an accurate exposure history of tetramine in the body.