Presented at the 55th Annual Meeting of the American Academy of Forensic Sciences, February 17–22, 2003, in Chicago, IL.
The Use of Crossover Immunoelectrophoresis to Detect Human Blood Protein in Soil from an Ambush Scene in Kosovo
Article first published online: 5 MAR 2012
2012 American Academy of Forensic Sciences. Published 2012. This article is a U.S. Government work and is in the public domain in the U.S.A.
Journal of Forensic Sciences
Volume 57, Issue 4, pages 873–879, July 2012
How to Cite
Tuller, H. and Saunders, R. (2012), The Use of Crossover Immunoelectrophoresis to Detect Human Blood Protein in Soil from an Ambush Scene in Kosovo. Journal of Forensic Sciences, 57: 873–879. doi: 10.1111/j.1556-4029.2012.02070.x
Supported in parts by grants from Sigma Xi, and the Museum of Natural Science, Louisiana State University.
- Issue published online: 2 JUL 2012
- Article first published online: 5 MAR 2012
- Received 1 Dec. 2010; and in revised form 25 April 2011; accepted 14 May 2011.
- forensic science;
- forensic archeology;
- blood protein;
Abstract: This study examines the survivability of human blood proteins in soils from a year and a half old ambush scene in Kosovo. A total of 72 soil samples were collected, a number of which were directly associated with bone fragments or bullet projectiles. The samples were examined using crossover immunoelectrophoresis (CIEP) to determine the presence of blood protein and species affiliation. Human blood proteins were identified in 44 of the 72 samples (61%) with the majority of the positive observations (29 of 44) found 0.0–4.5 cm below ground surface (65%). Chi-squared and two-sample difference of proportions tests confirmed significant differences between samples with and without associated physical evidence and the presence and depth of human blood proteins. While DNA has largely replaced immunological analysis in forensic analyses, our results suggest that in particular situations, CIEP may still be a valuable tool in criminology.