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Validation Study of KPICS SpermFinder™ by NicheVision Forensics, LLC for the Identification of Human Spermatozoa


  • Angela Mitchell M.S.

    1. Allegheny County Office of the Medical Examiner, Forensic Laboratory, Forensic Biology, 1520 Penn Avenue, Pittsburgh, PA 15222.
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  • Instrumentation purchased and validation study supported through National Institute of Justice FY 2008 Forensic DNA Unit Efficiency Improvement Program: 2008-DN-BX-K191. No conflicting interests or affiliations between the author or agency and NicheVision Forensics, LLC to report. The opinions, findings, and conclusions or recommendations expressed in this publication/program/exhibition are those of the author and do not necessarily reflect those of the Department of Justice.

Additional information reprints and requests:
Angela Mitchell, M.S.
Allegheny County Office of the Medical Examiner
Forensic Laboratory, Forensic Biology
1520 Penn Avenue
Pittsburgh, PA 15222


Abstract:  Microscopic analysis for the identification of spermatozoa is commonly performed during the forensic examination of sexual assault evidence. Two widely utilized methods for the confirmation of the presence of spermatozoa are visualization of the cells via phase-contrast microscopy with wet mounted samples and bright field microscopy with histologically stained samples. The KPICS SpermFinder™ by NicheVision Forensics, LLC accelerates this time-consuming process via an automated microscope with an algorithm designed to locate spermatozoa on a Christmas tree histologically stained microscope slide. Upon a qualified scientist’s review of the generated data, the KPICS SpermFinder™ was able to locate spermatozoa, typically finding on average 106.28% ± 115.37% more spermatozoa than with manual examinations. The KPICS SpermFinder™ provided the location of identified cells with reproducible results.