AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples

Authors


  • Presented in part at the 62nd Annual Scientific Meeting of the American Academy of Forensic Sciences, February 22-27, 2010, Seattle WA. Some of the data is also presented in the PrepFiler Express™ and PrepFiler Express BTA™ Forensic DNA Extraction Kit User Manual.

Additional information and reprint requests:
Lori K. Hennessy, Ph.D.
Life Technologies Corporation
850 Lincoln Centre Drive
Mail Stop 404-1
Foster City, CA 94404
E-mail: lori.hennessy@lifetech.com

Abstract

Abstract:  The AutoMate Express™ Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler™ lysis buffer or PrepFiler BTA™ lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep™ column, the lysate in the sample tubes were loaded onto AutoMate Express™ instrument and DNA was extracted using one of the two instrument extraction protocols. Our study showed that DNA was recovered from as little as 0.025 μL of blood. DNA extracted from casework-type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day-to-day operation.

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