Forensic Identification Using a Multiplex Assay of 47 SNPs

Authors

  • Yi-Liang Wei Ph.D.,

    1. Key Laboratory of Ministry of Public Health for Forensic Science, Department of Forensic Science, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China
    2. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, China
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  • Cai-Xia Li Ph.D.,

    1. Ministry of Public Security, Institute of Forensic Science, Beijing 100038, China
    2. Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing 100038, China
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  • Jing Jia Ph.D.,

    1. Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
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  • Lan Hu Ph.D.,

    1. Ministry of Public Security, Institute of Forensic Science, Beijing 100038, China
    2. Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing 100038, China
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  • Yao Liu Ph.D.

    1. Key Laboratory of Ministry of Public Health for Forensic Science, Department of Forensic Science, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China
    2. Ministry of Public Security, Institute of Forensic Science, Beijing 100038, China
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  • Supported by Basic Research Project (Grant 2007JB001) awarded by the Institute of Forensic Science, Ministry of Public Security, China.

  • <Correction added after online publication 26 April 2012: Two corresponding authors worked on this paper, but Dr. Lan Hu's address was mistakenly omitted from the original version of this paper. It has been added above.>

Additional information and reprint requests:
Dr. Yao Liu, Ph.D.
Ministry of Public Security
Institute of Forensic Science
Muxidi Nanli
Xicheng District
Beijing 100038
China
E-mail: liuyao1123@yahoo.cn

Dr. Lan Hu, Ph.D.
Ministry of Public Security
Institute of Forensic Science
Muxidi Nanli
Xicheng District
Beijing 100038
China
E-mail: hulan328@yahoo.com

Abstract

Abstract:  As a powerful alternative to short tandem repeat (STR) profiling, we have developed a novel panel of 47 single nucleotide polymorphisms (SNPs) for DNA profiling and ABO genotyping. We selected 42 of the 47 SNPs from a panel of 86 markers that were previously validated as universal individual identification markers and identified five additional SNPs including one gender marker and four ABO loci. Match probability of the 42 validated SNPs was found to be 9.5 × 10−18 in Han Chinese. SNP analysis correctly assessed a panel of historical cases, including both paternity identifications in trios and individual identifications. In addition, while STR profiling of degraded DNA provided information for 11 loci of 16 potential markers with low peak intensities, SNPstream® genotyping was sufficient to identify all 47 SNPs. In summary, SNP analysis is equally effective as STR profiling, but appears more suited for individual identification than STR profiling in cases where DNA may be degraded.

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