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Specificity, Sensitivity, and Operability of RSID™-Urine for Forensic Identification of Urine: Comparison with ELISA for Tamm-Horsfall Protein

Authors

  • Tomoko Akutsu Ph.D.,

    1. Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa 277-0882, Chiba, Japan.
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  • Ken Watanabe Ph.D.,

    1. Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa 277-0882, Chiba, Japan.
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  • Koichi Sakurada D.D.S., Ph.D.

    1. Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa 277-0882, Chiba, Japan.
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Additional information and reprint requests:
Tomoko Akutsu, Ph.D.
Third Biology Section
First Department of Forensic Science
National Research Institute of Police Science
6-3-1, Kashiwanoha
Kashiwa 277-0882
Chiba
Japan
E-mail: tomoko@nrips.go.jp

Abstract

Abstract:  In this study, the specificity, sensitivity, and operability of RSID™-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID™-Urine and ELISA detection of THP. The detection limit of RSID™-Urine equated to 0.5 μL of urine; although the sensitivity of RSID™-Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID™-Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID™-Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID™-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP.

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