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Table S1.Methylobacterium strains used in construction of fluorescent ancestors.

Table S2. One-tail t-tests of population fitness among generations 300, 900, and 1500.

Figure S1. Plasmid map of the insertional expression vector pHC10 used to generate labeled strains expressing the yellow fluorescent protein Venus.

Figure S2. The fitness and growth rates of 22 selected isolates from A and B populations were measured in their selected and nonselected environments.

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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.