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Figure S1. Female (A) and male (B) abdominal glands in Heliconius charithonia.

Figure S2. Phylogeny of Heliconius inferred from combined mitochondrial (Co and 16S) and nuclear data (Ef1α, dpp, ap and wg) modified from Beltrán et al. (2007).

Figure S3. Consensus trees obtained from bootstrap analysis with 2000 replicates on trees constructed by maximum parsimony using heuristic search with TBR swapping algorithm.

Figure S4. Divergence in chemical composition of blend from abdominal glands and phenotypic distance between pair of Heliconius species.

Table S1. Chemical composition of blends from male abdominal glands of 11 species of Heliconius.

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