File S1. A potential test for local adaptation.

Figure S1. Approximate Bayesian clustering (Pritchard et al. 2000) of S. latifolia individuals in the sample using X-linked DNA polymorphisms.

Table S1. Male S. latifolia plants from natural populations used in the study.

Table S2. Primers for PCR amplification of sex-linked genes.

Table S3. DNA polymorphism in the X- and Y-linked genes in several geographical regions not reported in Table 1 of the main text (Sets 5–10 defined in Table S1).

Table S4. Results of HKA tests comparing DNA polymorphism in the concatenated Y-linked genes between populations.

Table S5. Population subdivision statistics for the X- and Y-linked genes for sets not reported in Table 3 of the main text.

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