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Table S1. Primer sequences for RT-PCR reactions.

Table S2. Parameter estimates of binary models testing for differences between ancestral and evolved lines in sex ratios.

Table S3. Parameter estimates of binary models testing for differences between ancestral and evolved lines in the frequencies of hermaphrodites carrying visible eggs/oocytes.

Table S4. Parameter estimates of ordinal models testing for differences between ancestral and evolved lines in tail phenotypes.

Table S5. Parameter estimates (mean and 95% highest posterior density confidence interval) and P-values from the best-fitting model predicting relative expression levels of her-1 in wild-type and ancestral mutant lines (CT = Intercept + Genotype + Background + Interaction + e).

Table S6. Parameter estimates (mean and 95% highest posterior density confidence interval) and P-values from the best-fitting model predicting relative expression levels of tra-2 in wild-type and ancestral mutant lines (CT = Intercept + Genotype + e).

Table S7. Parameter estimates (mean and 95% highest posterior density confidence interval) and P-values from the best-fitting model predicting relative expression levels of tra-3 in wild-type and ancestral mutant lines (CT = Intercept + e).

Table S8. Parameter estimates (mean and 95% highest posterior density confidence interval) and P-values from the best-fitting model predicting relative expression levels of fem-3 in wild-type and ancestral mutant lines (CT = Intercept + Genotype + Background + Interaction + e).

Table S9. Parameter estimates (mean and 95% highest posterior density confidence interval) and P-values from the best-fitting model predicting relative expression levels of tra-1 in wild-type and ancestral mutant lines (CT = Intercept + Genotype + Background + e).

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