EXPERIMENTAL EVOLUTION OF ULTRAVIOLET RADIATION RESISTANCE IN ESCHERICHIA COLI

Authors

  • Robert P. Goldman,

    1. College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, California 91766
    2. E-mail: rgoldman@westernu.edu
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  • Michael Travisano

    1. College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, California 91766
    2. Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204
    3. Department of Ecology, Evolution and Behavior, 1987 Upper Buford Circle, University of Minnesota, St. Paul, Minnesota 55108
    4. E-mail: travisan@umn.edu
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Abstract

Ultraviolet (UV) light is a major cause of stress, mutation, and mortality in microorganisms, causing numerous forms of cellular damage. Nevertheless, there is tremendous variation within and among bacterial species in their sensitivity to UV light. We investigated direct and correlated responses to selection during exposure to UV. Replicate lines of Escherichia coli K12 were propagated for 600 generations, half with UV and half as a control without UV. All lines responded to selection, and we found strong positive and negative correlated responses to selection associated with increased UV resistance. Compared to Control populations, UV-selected populations increased in desiccation and starvation resistance approximately twofold but were 10 times more sensitive to hypersalinity. There was little evidence for a persistent large competitive fitness cost to UV resistance. These results suggest that natural variation in UV resistance may be maintained by trade-offs for resistance to other abiotic sources of mortality. We observed an average twofold increase in cell size by the UV-selected populations, consistent with a structural mode of adaptation to UV exposure having preadaptive and maladaptive consequences to other abiotic stresses.

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