EXPERIMENTAL EVOLUTION OF ULTRAVIOLET RADIATION RESISTANCE IN ESCHERICHIA COLI
Article first published online: 20 SEP 2011
© 2011 The Author(s). Evolution© 2011 The Society for the Study of Evolution.
Volume 65, Issue 12, pages 3486–3498, December 2011
How to Cite
Goldman, R. P. and Travisano, M. (2011), EXPERIMENTAL EVOLUTION OF ULTRAVIOLET RADIATION RESISTANCE IN ESCHERICHIA COLI. Evolution, 65: 3486–3498. doi: 10.1111/j.1558-5646.2011.01438.x
- Issue published online: 1 DEC 2011
- Article first published online: 20 SEP 2011
- Accepted manuscript online: 11 AUG 2011 07:47PM EST
- Received May 25, 2009, Accepted April 12, 2011
Ultraviolet (UV) light is a major cause of stress, mutation, and mortality in microorganisms, causing numerous forms of cellular damage. Nevertheless, there is tremendous variation within and among bacterial species in their sensitivity to UV light. We investigated direct and correlated responses to selection during exposure to UV. Replicate lines of Escherichia coli K12 were propagated for 600 generations, half with UV and half as a control without UV. All lines responded to selection, and we found strong positive and negative correlated responses to selection associated with increased UV resistance. Compared to Control populations, UV-selected populations increased in desiccation and starvation resistance approximately twofold but were 10 times more sensitive to hypersalinity. There was little evidence for a persistent large competitive fitness cost to UV resistance. These results suggest that natural variation in UV resistance may be maintained by trade-offs for resistance to other abiotic sources of mortality. We observed an average twofold increase in cell size by the UV-selected populations, consistent with a structural mode of adaptation to UV exposure having preadaptive and maladaptive consequences to other abiotic stresses.