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Figure S1. NewHybrids classification of hybrid class of individuals located in the hybrid zone center.

Figure S2. A map of Lake Barrine (shown in light gray), with individual hybrid indices shown in bold.

Figure S3. Cline width (in demes) at a neutral locus, FIS at a neutral locus, and Rij at a neutral locus for a range of values for selection against hybrids and migration rates.

Figure S4. Cline width (in demes) at a neutral locus, FIS at a neutral locus, and Rij at a neutral locus for a range of values for selection against hybrids and strength of assortative mating.

Figure S5. A cartoon schematic of how simulations were conducted.

Table S1. Sampling points for this study.

Table S2. Loci used in this study, including their diagnostic SNPs and cutting patterns with listed restriction enzyme.

Table S3. Parameters for simulation for this study.

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