FITNESS OF ARABIDOPSIS THALIANA MUTATION ACCUMULATION LINES WHOSE SPONTANEOUS MUTATIONS ARE KNOWN
Version of Record online: 21 FEB 2012
© 2012 The Author(s).
Volume 66, Issue 7, pages 2335–2339, July 2012
How to Cite
Rutter, M. T., Roles, A., Conner, J. K., Shaw, R. G., Shaw, F. H., Schneeberger, K., Ossowski, S., Weigel, D. and Fenster, C. B. (2012), FITNESS OF ARABIDOPSIS THALIANA MUTATION ACCUMULATION LINES WHOSE SPONTANEOUS MUTATIONS ARE KNOWN. Evolution, 66: 2335–2339. doi: 10.1111/j.1558-5646.2012.01583.x
- Issue online: 3 JUL 2012
- Version of Record online: 21 FEB 2012
- Accepted manuscript online: 1 FEB 2012 02:35PM EST
- Received December 1, 2011, Accepted December 30, 2011, Data Archived: Dryad doi:10.5061/dryad.rk5fp6n9
- Genetic variation;
- G × E;
- quantitative genetics
Despite the fundamental importance of mutation to the evolutionary process, we have little knowledge of the direct consequences of specific spontaneous mutations to the fitness of the organism. Combining results of whole-genome sequencing with repeated field assays of survival and reproduction, we quantify the combined effects on fitness of spontaneous mutations identified in Arabidopsis thaliana. We demonstrate that the effects are beneficial, deleterious, or neutral depending on the environmental context. Some lines, bearing mutations disrupting known loci, differ strongly in fitness from the founder or premutation genotype. Those effects vary across environments, for example, a line with a major deletion spanning a transcription factor gene expressed lower fitness than the founder under most conditions but exceeded the founder's fitness in one environment. The large contribution of genotype by environment interaction (G × E) to mutation effects on fitness implies spatial and/or temporal variation in selection on new mutations and could contribute to the maintenance of standing genetic variation.