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Table S1. Accession information for 11 Nolana study species.

Table S2. Prezygotic (pollen-pistil) isolation indices for all species pairs.

Table S3. Postzygotic isolation (fruit set/pollination) indices for all species pairs.

Table S4. Postzygotic isolation (mericarp size) indices for all species pairs.

Table S5. Postzygotic isolation (seed set) indices for all species pairs.

Table S6. Estimated "rates" of evolution of different stages of reproductive isolation.

Table S7. Estimated "rates" of evolution of allopatric (nonoverlapping) versus sympatric (overlapping) pairs, at four different stages of reproductive isolation.

Table S8. Results of partial Mantel tests of the relationship between reproductive isolation and both genetic and geographical distance, for each of four isolation stages.

Table S9. Species means for floral traits.

Table S10. Relationship between genetic and geographic distance, and floral trait differences.

Figure S1. Relationship between geographical distance and each of (A) postmating prezygotic isolation; (B) postmating isolation estimated from fruit set/pollination; (C) postmating isolation estimated from mericarp size; (D) postmating isolation estimated from F1 seed set. Statistics for each relationship are shown in Table 2.

Figure S2. Relationship between pairwise species differences in flower size (corolla diameter) and (A) genetic distance (Cor = 0.513, Mantel test P = 0.0039; Pearson's P = 0.000314), and (B) geographic distance (Cor = 0.322, Mantel test P = 0.0567; Pearson's P = 0.0309).

Figure S3. Relationship between pairwise species differences in style length and postmating prezygotic isolation index (Cor = -0.0845, Mantel test P = 0.808; Pearson's P = 0.765).

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