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Table S1. Sequences of all primers used during this study, either for microsatellite loci (Sy_ty_1 -- Sy_ty_24) or for genotyping the bacteria of the genus Vibrio spp. (pyrH, recA, 16S rDNA).

Table S2. All isolated Vibrio phylotypes from the four different sampling locations (D: Denmark, I: Italy, K: Germany, S: Sweden) are displayed here.

Table S3. Reference Vibrio sequences used for the phylogeny. The references sequences were aligned to the sequences of the particular gene (16S rDNA, pyrH, recA).

Table S4. SIMPER results: Numbers show similarities in percentages of Vibrio phylotypes within and between locations.

Table S5. Displays results of single ANOVAs testing for differences in the antimicrobial activity against several Vibrio phylotypes for plasma from the different pipefish locations.

Figure S1. The haplotype network for rec A shows the proportion of sequences from Sweden in red, from Italy in blue, from Denmark in yellow, and the proportion of German sequences is displayed in green.

Figure S2. The haplotype network for pyr H shows the proportion of sequences from Sweden in red, from Italy in blue, from Denmark in yellow, and the proportion of German sequences is displayed in green.

Figure S3. The haplotype network for 16S rDNA shows the proportion of sequences from Sweden in red, from Italy in blue, from Denmark in yellow, and the proportion of German sequences is displayed in green.

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