A SIGNIFICANT COMPONENT OF AGEING (DNA DAMAGE) IS REFLECTED IN FADING BREEDING COLORS: AN EXPERIMENTAL TEST USING INNATE ANTIOXIDANT MIMETICS IN PAINTED DRAGON LIZARDS
Article first published online: 9 APR 2012
© 2012 The Author(s). Evolution© 2012 The Society for the Study of Evolution.
Volume 66, Issue 8, pages 2475–2483, August 2012
How to Cite
Olsson, M., Tobler, M., Healey, M., Perrin, C. and Wilson, M. (2012), A SIGNIFICANT COMPONENT OF AGEING (DNA DAMAGE) IS REFLECTED IN FADING BREEDING COLORS: AN EXPERIMENTAL TEST USING INNATE ANTIOXIDANT MIMETICS IN PAINTED DRAGON LIZARDS. Evolution, 66: 2475–2483. doi: 10.1111/j.1558-5646.2012.01617.x
- Issue published online: 26 JUL 2012
- Article first published online: 9 APR 2012
- Accepted manuscript online: 13 MAR 2012 11:20AM EST
- Received July 21, 2011 , Accepted January 13, 2012 , Data Archived: Dryad doi:10.5061/dryad.bb5dr437
A decade ahead of their time, von Schantz et al. united sexual selection and free radical biology by identifying causal links between deep-rooted physiological processes that dictate resistance to toxic waste from oxidative metabolism (reactive oxygen species, ROS), and phenotypic traits, such as ornaments. Ten years later, these ideas have still only been tested with indirect estimates of free radical levels (oxidative stress) subsequent to the action of innate and dietary antioxidants. Here, we measure net superoxide (a selection pressure for antioxidant production) and experimentally manipulate superoxide antioxidation using a synthetic mimetic of superoxide dismutase (SOD), Eukarion 134 (EUK). We then measure the toxic effect of superoxide in terms of DNA erosion and concomitant loss of male breeding coloration in the lizard, Ctenophorus pictus. Control males suffered more DNA damage than EUK males. Spectroradiometry showed that male coloration is lost in relation to superoxide and covaries with DNA erosion; in control males, these variables explained loss of color, whereas in EUK males, the fading of coloration was unaffected by superoxide and unrelated to DNA damage. Thus, EUK's powerful antioxidation removes the erosion effect of superoxide on coloration and experimentally verifies the prediction that colors reflect innate capacity for antioxidation.