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Table S1. Mean fitness effect of each mutation on each host.

Figure S1. (A) Incubation times on each host were determined based on the time it took the wild-type φX174 to reach at least 106 pfu, after incubating host cultures with ∼104 pfu.

Figure S2. qPCR standard curve.

Figure S3. Lethality on solid medium.

Figure S4. Measuring fitness.

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