GENETIC EXCHANGES OF INTEINS BETWEEN PRASINOVIRUSES (PHYCODNAVIRIDAE)
Article first published online: 10 AUG 2012
© 2012 The Author(s). Evolution© 2012 The Society for the Study of Evolution.
Volume 67, Issue 1, pages 18–33, January 2013
How to Cite
Clerissi, C., Grimsley, N. and Desdevises, Y. (2013), GENETIC EXCHANGES OF INTEINS BETWEEN PRASINOVIRUSES (PHYCODNAVIRIDAE). Evolution, 67: 18–33. doi: 10.1111/j.1558-5646.2012.01738.x
- Issue published online: 4 JAN 2013
- Article first published online: 10 AUG 2012
- Accepted manuscript online: 21 JUL 2012 09:00AM EST
- Received April 3, 2012, Accepted June 29, 2012
- Gene conversion;
- lateral gene transfer;
Phylogenetic diversity in the Phycodnaviridae (double-stranded DNA viruses infecting photosynthetic eukaryotes) is most often studied using their DNA polymerase gene (PolB). This gene and its translated protein product can harbor a selfish genetic element called an “intein” that disrupts the sequence of the host gene without affecting its activity. After translation, the intein peptide sequence self-excises precisely, producing a functional ligated host protein. In addition, inteins can encode homing endonuclease (HEN) domains that permit the possibility of lateral transfers to intein-free alleles. However, no clear evidence for their transfer between viruses has previously been shown. The objective of this paper was to determine whether recent transfers of inteins have occurred between prasinoviruses (Phycodnaviridae) that infect the Mamiellophyceae, an abundant and widespread class of unicellular green algae, by using DNA sequence analyses and cophylogenetic methods. Our results suggest that transfer among prasinoviruses is a dynamic ongoing process and, for the first time in the Phycodnaviridae family, we showed a recombination event within an intein.