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Table S1. Coordinates for sample sites.

Table S2. Mixed model ANOVA for rhizobial isolates from Acmispon wrangelianus originating from serpentine and nonserpentine soils at three reserves, grown with and without excess nickel.

Table S3. Mixed model ANOVAs for rhizobial isolates from Acmispon wrangelianus (Aw) and Medicago polymorpha (Mp) originating from serpentine and nonserpentine soils at McLaughlin, grown with and without excess nickel.

Figure S1. Locations of the reserves sampled (stars).

Figure S2. The relationship between nickel tolerance and fitness in the absence of nickel for rhizobial isolates from: (A) Acmispon wrangelianus (Aw) at Jasper Ridge, (B) Aw at Hopland, (C) Aw at McLaughlin, and (D) Medicago polymorpha (Mp) at McLaughlin.

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