This study explored relationships between perceptions of intra-team communication and role ambiguity within an interdependent team sport environment. Participants were 116 intercollegiate-level team sport athletes (M age = 20.6 years). For males, the intra-team communication dimension of acceptance was found to predict the majority of role ambiguity dimensions for both performance contexts of offense and defense (.09 ≤ R2adj. ≤ .16). However, limited support for this relationship was found for females, as acceptance communication predicted only one role ambiguity dimension (evaluation of role performance on defense). These findings have implications for applied practice in terms of reducing group members’ perceptions of role ambiguity in interdependent team sport.