To explore the theory that some men experience antigay bias because of a defensive reaction to uncertainties surrounding their own sexuality (cf. Herek, 1987), we conducted a study (n = 132 men) in which we physiologically measured the affective underpinnings of antigay bias while individuals with different levels of self-reported bias viewed sexually explicit material. Those higher in antigay bias exhibited less positive affect than did others, but did not appear to experience a defensive reaction. Given these results, combined with those of our previous studies, we conclude that evidence for the existence of a group of men who exhibit antigay bias because of a hidden or unconscious attraction toward men is difficult to demonstrate using current methodology.