This study was designed to examine the extent to which masculine gender-role stress, sexual prejudice, and antigay anger collectively facilitate antigay aggression. Participants were 135 heterosexual men who completed a structured interview assessing masculine gender-role stress, sexual prejudice, anger in response to a vignette depicting a non-erotic male–male intimate relationship (i.e., partners holding hands, kissing), and past perpetration of antigay aggression. The results indicate that the association between masculine gender-role stress and antigay aggression is partially mediated by antigay anger among sexually prejudiced men. These findings contribute to theoretical understanding of antigay aggression. Implications for future research and intervention are discussed.