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Reoxidation of cytosolic NADPH in Kluyveromyces lactis
Article first published online: 28 NOV 2005
DOI: 10.1111/j.1567-1364.2005.00021.x
1567-1364/asset/cover.gif?v=1&s=a7ff0154658a7d375178c0feb32e2c54f157b9a1 "FEMS Yeast Research")
Additional Information
How to Cite
Tarrío, N., Becerra, M., Cerdán, M. E. and Siso, M. I. G. (2006), Reoxidation of cytosolic NADPH in Kluyveromyces lactis. FEMS Yeast Research, 6: 371–380. doi: 10.1111/j.1567-1364.2005.00021.x
Publication History
- Issue published online: 19 APR 2006
- Article first published online: 28 NOV 2005
- Received 28 April 2005; revised 21 June 2005; accepted 21 June 2005.First published online 28 November 2005.
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Keywords:
- NADPH reoxidation;
- Kluyveromyces lactis;
- rag2 mutant;
- alternative mitochondrial dehydrogenases;
- oxidative stress
Abstract
Saccharomyces cerevisiae and Kluyveromyces lactis are considered to be the prototypes of two distinct metabolic models of facultatively-aerobic yeasts: Crabtree-positive/fermentative and Crabtree-negative/respiratory, respectively. Our group had previously proposed that one of the molecular keys supporting this difference lies in the mechanisms involved in the reoxidation of the NADPH produced as a consequence of the activity of the pentose phosphate pathway. It has been demonstrated that a significant part of this reoxidation is carried out in K. lactis by mitochondrial external alternative dehydrogenases which use NADPH, the enzymes of S. cerevisiae being NADH-specific. Moreover, the NADPH-dependent pathways of response to oxidative stress appear as a feasible alternative that might co-exist with direct mitochondrial reoxidation.