Editor: Lex Scheffers
The genome-wide screening of yeast deletion mutants to identify the genes required for tolerance to ethanol and other alcohols
Article first published online: 2 FEB 2006
FEMS Yeast Research
Volume 6, Issue 5, pages 744–750, August 2006
How to Cite
Fujita, K., Matsuyama, A., Kobayashi, Y. and Iwahashi, H. (2006), The genome-wide screening of yeast deletion mutants to identify the genes required for tolerance to ethanol and other alcohols. FEMS Yeast Research, 6: 744–750. doi: 10.1111/j.1567-1364.2006.00040.x
- Issue published online: 11 JUL 2006
- Article first published online: 2 FEB 2006
- Received 7 August 2005; revised 31 October 2005; accepted 3 November 2005.First published online 2 February 2006.
- yeast deletion clones;
- Saccharomyces cerevisiae
A set of homozygous diploid deletion mutants of the yeast Saccharomyces cerevisiae was screened for the genes required for tolerance to aliphatic alcohols. The screen identified 137, 122 and 48 deletion mutants sensitive to ethanol, 1-propanol and 1-pentanol, respectively. A number of the genes required for ethanol tolerance were those also required for tolerance to other alcohols. Numerous mutants with defective genes encoding for vacuolar H+-ATPase (V-ATPase) were cosensitive to these alcohols. A global screening approach of yeast deletion library mutants was useful in elucidating the mechanisms of alcohol tolerance based on different lipophilicities.