Editor: Monique Bolotin-Fukuhara
Heterologous protein production in the yeast Kluyveromyces lactis
Article first published online: 21 MAR 2006
FEMS Yeast Research
Volume 6, Issue 3, pages 381–392, May 2006
How to Cite
Van Ooyen, A. J.J., Dekker, P., Huang, M., Olsthoorn, M. M.A., Jacobs, D. I., Colussi, P. A. and Taron, C. H. (2006), Heterologous protein production in the yeast Kluyveromyces lactis. FEMS Yeast Research, 6: 381–392. doi: 10.1111/j.1567-1364.2006.00049.x
- Issue published online: 19 APR 2006
- Article first published online: 21 MAR 2006
- Received 4 May 2005; revised 4 November 2005; accepted 7 November 2005.First published online 21 March 2006.
- Kluyveromyces lactis;
- protein synthesis
Kluyveromyces lactis is both scientifically and biotechnologically one of the most important non-Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well-known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression.