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Characterization of natural hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum

  1. Christine Le Jeune1,
  2. Marc Lollier1,
  3. Catherine Demuyter1,
  4. Claude Erny1,
  5. Jean-Luc Legras2,
  6. Michel Aigle3,
  7. Isabelle Masneuf-Pomarède4

Article first published online: 16 FEB 2007

DOI: 10.1111/j.1567-1364.2007.00207.x

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 7, Issue 4, pages 540–549, June 2007

Additional Information

How to Cite

Le Jeune, C., Lollier, M., Demuyter, C., Erny, C., Legras, J.-L., Aigle, M. and Masneuf-Pomarède, I. (2007), Characterization of natural hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. FEMS Yeast Research, 7: 540–549. doi: 10.1111/j.1567-1364.2007.00207.x

Author Information

  1. 1

    Laboratoire Vigne Biotechnologie et Environnement, Université de Haute-Alsace, Herrlisheim, Colmar, France

  2. 2

    Institut national de la Recherche Agronomique (INRA) UMR SVQV Œnologie, Herrlisheim, Colmar, France

  3. 3

    UMR 5122, Université de Lyon 1, Villeurbanne, France

  4. 4

    ENITA Bordeaux, Gradignan, France

*Correspondence: Le Jeune Christine, Laboratoire Vigne Biotechnologie et Environnement, Université de Haute-Alsace, 32 rue de Herrlisheim, BP 568, 68 008 Colmar Cedex, France. Tel.: + 00 33 3 89 30 31 36; fax: + 00 33 3 89 30 31 36; e-mail: christine.le-jeune@uha.fr

  1. Editor: Cletus Kurtzman

Publication History

  1. Issue published online: 16 FEB 2007
  2. Article first published online: 16 FEB 2007
  3. Received 13 April 2006; revised 7 December 2006; accepted 7 December 2006.First published online 15 February 2007.

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Keywords:

  • Saccharomyces cerevisiae;
  • Saccharomyces bayanus var. uvarum;
  • hybrid cells

Abstract

Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR–restriction fragment length polymorphism (RFLP) of the MET2 gene, δ-PCR, and microsatellite patterns. Karyotypes and MET2 fragments of the nine strains corresponded to mixed chromosomal bands and restriction patterns for both Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. They also responded positively to amplification with microsatellite primers specific to both species and were demonstrated to be diploid. However, meiosis led to absolute nonviability of their spores on complete medium. All the results demonstrated that the nine yeast strains isolated were S. cerevisiae×S. bayanus var. uvarum diploid hybrids. Moreover, microsatellite DNA analysis identified strains isolated in the same cellar as potential parents belonging to S. bayanus var. uvarum and S. cerevisiae.

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