Editor: Monique Bolotin-Fukuhara
Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex
Article first published online: 27 JUL 2007
FEMS Yeast Research
Volume 7, Issue 8, pages 1237–1247, December 2007
How to Cite
Fekete, V., Čierna, M., Poláková, S., Piškur, J. and Sulo, P. (2007), Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex. FEMS Yeast Research, 7: 1237–1247. doi: 10.1111/j.1567-1364.2007.00287.x
- Issue published online: 27 JUL 2007
- Article first published online: 27 JUL 2007
- Received 8 December 2006; revised 29 May 2007; accepted 29 May 2007.First published online 27 July 2007.
- Petite mutation;
- Saccharomyces/Kluyveromyces complex;
- ethidium bromide;
Petite-positivity – the ability to tolerate the loss of mtDNA – was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed ‘moot’ phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.