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Ecm11 protein of yeast Saccharomyces cerevisiae is regulated by sumoylation during meiosis

  1. Apolonija Bedina Zavec1,
  2. Aleksandra Comino1,
  3. Metka Lenassi2,
  4. Radovan Komel1

Article first published online: 20 SEP 2007

DOI: 10.1111/j.1567-1364.2007.00307.x

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 8, Issue 1, pages 64–70, February 2008

Additional Information

How to Cite

Zavec, A. B., Comino, A., Lenassi, M. and Komel, R. (2008), Ecm11 protein of yeast Saccharomyces cerevisiae is regulated by sumoylation during meiosis. FEMS Yeast Research, 8: 64–70. doi: 10.1111/j.1567-1364.2007.00307.x

Author Information

  1. 1

    Laboratory for Biosynthesis and Biotransformation, National Institute of Chemistry, Hajdrihova, Ljubljana, Slovenia

  2. 2

    Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov Trg, Ljubljana, Slovenia

*Correspondence: Apolonija Bedina Zavec, Laboratory for Biosynthesis and Biotransformation, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia. Tel.: +386 1 476 0200; fax: +386 1 476 0300; e-mail: polona.bedina@ki.si

  1. Editor: Ian Dawes

Publication History

  1. Issue published online: 20 SEP 2007
  2. Article first published online: 20 SEP 2007
  3. Received 15 March 2007; revised 19 July 2007; accepted 26 July 2007.First published online 20 September 2007.

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Keywords:

  • SUMO;
  • meiosis;
  • Ecm11

Abstract

Damaged regulation of the small ubiquitin–like modifier (SUMO) system contributes to some human diseases; therefore, it is very important to identify the SUMO targets and to determine the function of their sumoylation. In this study, it is shown that Ecm11 protein in Saccharomyces cerevisiae is modified by SUMO during meiosis. It is known that Ecm11 is required in the early stages of yeast meiosis where its function is related to DNA replication and crossing over. Here it is shown that the level of Ecm11 protein is low in mitosis, but high in meiosis. The highest level of Ecm11 is in the early-middle phase of sporulation. A specific site of sumoylation was identified in Ecm11 at Lys5 and evidence is provided that sumoylation at this site directly regulates Ecm11 function in meiosis. On the other hand, no relationship was observed between sumoylation of Ecm11 and its role during vegetative growth. It was shown that Ecm11 interacts with Siz2 SUMO ligase in a two-hybrid system; although Siz2 is not essential for the Ecm11 sumoylation.

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