Present address: Claudia Fischer, DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Cultures, Braunschweig, Germany.
Posttranscriptional regulation of FLO11 upon amino acid starvation in Saccharomyces cerevisiae
Article first published online: 12 NOV 2007
© 2007 Federation of European Microbiological Societies
FEMS Yeast Research
Volume 8, Issue 2, pages 225–236, March 2008
How to Cite
Fischer, C., Valerius, O., Rupprecht, H., Dumkow, M., Krappmann, S. and Braus, G. H. (2008), Posttranscriptional regulation of FLO11 upon amino acid starvation in Saccharomyces cerevisiae. FEMS Yeast Research, 8: 225–236. doi: 10.1111/j.1567-1364.2007.00331.x
Editor: Isak Pretorius
- Issue published online: 12 NOV 2007
- Article first published online: 12 NOV 2007
- Received 13 June 2007; revised 25 September 2007; accepted 3 October 2007.First published online 12 November 2007.
- amino acid starvation;
- FLO11 expression;
Various starvation conditions cause adhesive growth of haploid cells or pseudohyphae formation of diploid cells of Saccharomyces cerevisiae. For the genetic Σ1278b background, these morphological changes depend on the expression of the gene encoding the cell wall glycoprotein Flo11p, which is increased during nutritional limitations. Deletion of the genes encoding the transcriptional coactivators Rsc1p or Gcn5p impairs FLO11 transcription, which consequently leads to a loss of both haploid invasive growth and diploid pseudohyphae development upon glucose and nitrogen limitation, respectively. In contrast, amino acid starvation induces FLO11-dependent adhesive growth of the rsc1Δ and gcn5Δ strains although FLO11 transcription remains very low. The double deletion strain rsc1Δflo11Δ, however, does not grow adhesively, suggesting that the adhesion of the rsc1Δ strain at amino acid starvation is still FLO11-dependent. The FLO11prom-lacZ-encoded β-galactosidase activities of the rsc1Δ and gcn5Δ mutant strains increase manifold upon amino acid starvation. It is therefore concluded that low levels of FLO11 transcripts are essential and sufficient for derepression of FLO11 expression and adhesive growth during amino acid starvation. A posttranscriptional control is assumed to be behind this phenomenon that permits the increased FLO11 expression from low FLO11 transcript abundances.