Editor: Terrance Cooper
RESEARCH ARTICLE: Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor
Article first published online: 16 JAN 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Yeast Research
Volume 8, Issue 3, pages 386–399, May 2008
How to Cite
Fong, C. S., Temple, M. D., Alic, N., Chiu, J., Durchdewald, M., Thorpe, G. W., Higgins, V. J. and Dawes, I. W. (2008), RESEARCH ARTICLE: Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor. FEMS Yeast Research, 8: 386–399. doi: 10.1111/j.1567-1364.2007.00349.x
- Issue published online: 30 JAN 2008
- Article first published online: 16 JAN 2008
- Received 21 August 2007; revised 4 December 2007; accepted 6 December 2007.First published online 16 January 2008.
- oxidative stress;
- lipid peroxidation;
- cell-cycle delay
Cells treated with low doses of linoleic acid hydroperoxide (LoaOOH) exhibit a cell-cycle delay that may provide a mechanism to overcome oxidative stress. Strains sensitive to LoaOOH from the genome-wide deletion collection were screened to identify deletants in which the cell-cycle delay phenotype was reduced. Forty-seven deletants were identified that were unable to mount the normal delay response, implicating the product of the deleted gene in the oxidant-mediated cell-cycle delay of the wild-type. Of these genes, SWI6 was of particular interest due to its role in cell-cycle progression through Start. The swi6 deletant strain was delayed on entry into the cell cycle in the absence of an oxidant, and oxidant addition caused no further delay. Transforming the swi6 deletant with SWI6 on a plasmid restored the G1 arrest in response to LoaOOH, indicating that Swi6p is involved in oxidant sensing leading to cell division delay. Micro-array studies identified genes whose expression in response to LoaOOH depended on SWI6. The screening identified 77 genes that were upregulated in the wild-type strain and concurrently downregulated in the swi6 deletant treated with LoaOOH. These data show that functions such as heat shock response, and glucose transport are involved in the response.