You have full text access to this OnlineOpen article

Oxygen is required to restore flor strain viability and lipid biosynthesis under fermentative conditions

  1. Giacomo Zara1,
  2. Daniele Angelozzi2,
  3. Simona Belviso3,
  4. Laura Bardi4,
  5. Paola Goffrini5,
  6. Tiziana Lodi5,
  7. Marilena Budroni1,
  8. Ilaria Mannazzu1,2

Article first published online: 7 JAN 2009

DOI: 10.1111/j.1567-1364.2008.00472.x

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 9, Issue 2, pages 217–225, March 2009

Additional Information

How to Cite

Zara, G., Angelozzi, D., Belviso, S., Bardi, L., Goffrini, P., Lodi, T., Budroni, M. and Mannazzu, I. (2009), Oxygen is required to restore flor strain viability and lipid biosynthesis under fermentative conditions. FEMS Yeast Research, 9: 217–225. doi: 10.1111/j.1567-1364.2008.00472.x

Author Information

  1. 1

    Dipartimento di Scienze Ambientali Agrarie e Biotecnologie Agroalimentari, Sezione di Microbiologia Generale ed Applicata, Università degli Studi di Sassari, Sassari, Italy

  2. 2

    Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Ancona, Italy

  3. 3

    Dipartimento di Chimica Generale Organica e Applicata, Università degli Studi di Torino, Torino, Italy

  4. 4

    C.R.A. Centro di ricerca per lo studio delle relazioni fra pianta e suolo, Gruppo di ricerca di Torino, Torino, Italy; and

  5. 5

    Dipartimento di Genetica, Antropologia, Evoluzione, Università degli Studi di Parma, Parma, Italy

*Correspondence: Ilaria Mannazzu, Dipartimento di Scienze Ambientali Agrarie e Biotecnologie Agroalimentari, Sezione di Microbiologia Generale ed Applicata, Università degli Studi di Sassari, Viale Italia 39, Sassari 07100, Italy. Tel.: +39 079 229314; fax: +39 079 229370; e-mail: i.mannazzu@uniss.it

  1. Editor: Guenther Daum

Publication History

  1. Issue published online: 2 FEB 2009
  2. Article first published online: 7 JAN 2009
  3. Received 12 August 2008; revised 7 November 2008; accepted 10 November 2008.First published online 7 January 2009.

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (533K)

Keywords:

  • Saccharomyces cerevisiae;
  • flor yeast;
  • lipid biosynthesis;
  • fermentation;
  • real-time PCR

Abstract

To further elucidate the biosynthesis of lipids in flor strains under fermentative conditions, the transcription levels of the lipid biosynthetic genes ACS1, ACS2, ACC1, OLE1, ERG1, ERG11, ARE1 and ARE2, as well as the lipid composition and cell viability of a flor strain were compared with that of a non-flor strain during hypoxic and aerobic fermentations in the absence of lipid nutrients. While no significant differences in transcription levels or lipid compositions were observed between the two strains when oxygen was not limiting, significant differences were seen during hypoxic fermentation. In this last condition, the flor strain, in spite of higher levels of transcription of hypoxic genes, lost the abilities to desaturate fatty acids and complete ergosterol biosynthesis, and showed a dramatic loss of viability. In contrast, the non-flor strain, which showed lower transcription levels, was able to reach a balanced lipid composition and maintained a higher cell viability. One possible explanation is that the flor strain requires a higher amount of oxygen than the non-flor strain in order to carry out the oxygen-dependent steps of lipid biosynthesis under fermentative conditions.

View Full Article (HTML) Get PDF (533K)