Editor: Teun Boekhout
Screening of yeast strains for phytase activity
Article first published online: 10 MAR 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Yeast Research
Volume 9, Issue 3, pages 478–488, May 2009
How to Cite
Olstorpe, M., Schnürer, J. and Passoth, V. (2009), Screening of yeast strains for phytase activity. FEMS Yeast Research, 9: 478–488. doi: 10.1111/j.1567-1364.2009.00493.x
- Issue published online: 8 APR 2009
- Article first published online: 10 MAR 2009
- Received 18 September 2008; revised 14 January 2009; accepted 16 January 2009.First published online 10 March 2009.
- specific enzyme activity
A screening method was developed to elucidate the ability of different yeast strains to utilize phytic acid as sole phosphorus source. The growth test in liquid culture in a microtiter plate with phytic acid as sole phosphorus source was shown to be a reliable, fast and easy-to-use screening method. We tested 122 strains from 61 species with our method and observed growth differences among species and strains that were not detectable on solid medium. Specific phytase activities were measured for 10 yeasts strains, selected due to their strong growth in the liquid medium. Strains of Arxula adeninivorans and Pichia anomala reached the highest volumetric phytase activities. Arxula adeninivorans also displayed the highest intra- and extracellular specific activities. There were large differences in both extra- and intracellular phytase activities among species. Strain-specific extracellular phytase activities were detected in P. anomala. The presence of free phosphate in the media completely suppressed the extracellular phytase activity and also reduced intracellular phytase activity for all tested yeast strains.