An atypical PMR2  locus is responsible for hypersensitivity to sodium and lithium cations in the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D

Authors

  • Pascale Daran-Lapujade,

    1. Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands; and
    Search for more papers by this author
  • Jean-Marc Daran,

    1. Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands; and
    Search for more papers by this author
  • Marijke A.H. Luttik,

    1. Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands; and
    Search for more papers by this author
  • Marinka J.H. Almering,

    1. Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands; and
    Search for more papers by this author
  • Jack T. Pronk,

    1. Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands; and
    Search for more papers by this author
  • Peter Kötter

    1. Institut für Molekulare Biowissenschaften, J.W. Goethe Universität Frankfurt, Frankfurt, Germany
    Search for more papers by this author

  • Editor: Monique Bolotin-Fukuhara

Correspondence: Pascale Daran-Lapujade, Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands. Tel.: +31 15 278 9965; fax: +31 15 278 2355; e-mail: p.a.s.daran-lapujade@tudelft.nl

Abstract

Saccharomyces cerevisiae strains belonging to the CEN.PK family are widely used in fundamental and applied yeast research. These strains have been reported to be hypersensitive to sodium ions and a previous microarray-based genotyping study indicated an atypical organization of the PMR2 locus. In other S. cerevisiae strains, this locus harbours one to five ENA genes that encode plasma membrane sodium-pumping ATPases. Sequence analysis of the PMR2 locus in S. cerevisiae CEN.PK113-7D revealed the presence of a new ENA gene that showed substantial sequence differences, both at the nucleotide level and at the predicted amino acid sequence level, with previously described ENA genes. The presence of this single and atypical ENA gene correlated with hypersensitivity to sodium and, in particular, to lithium ions. The native ENA6 gene was transcriptionally induced by sodium and lithium ions, but, apparently, the capacity for sodium export upon full induction was insufficient to achieve the levels of sodium and lithium ion tolerance observed in other S. cerevisiae strains. The sodium and lithium hypersensitivity of CEN.PK strains, which is potentially detrimental during cultivation in sodium-rich media, could, however, be suppressed by overexpression of ENA6.

Ancillary