Editor: Terrance Cooper
Ume6p is required for germination and early colony development of yeast ascospores
Article first published online: 9 NOV 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Yeast Research
Volume 11, Issue 1, pages 104–113, February 2011
How to Cite
Strich, R., Khakhina, S. and Mallory, M. J. (2011), Ume6p is required for germination and early colony development of yeast ascospores. FEMS Yeast Research, 11: 104–113. doi: 10.1111/j.1567-1364.2010.00696.x
Present address: Michael J. Mallory, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
- Issue published online: 14 JAN 2011
- Article first published online: 9 NOV 2010
- Accepted manuscript online: 20 OCT 2010 10:35AM EST
- Received 21 May 2010; revised 5 October 2010; accepted 7 October 2010.Final version published online 9 November 2010.
- transcriptional repressor;
- cell cycle
Ume6p is a nonessential transcription factor that represses meiotic gene expression during vegetative growth in budding yeast. To relieve this repression, Ume6p is destroyed as cells enter meiosis and is not resynthesized until spore wall assembly. The present study reveals that spores derived from a ume6 null homozygous diploid fail to germinate. In addition, mutant spores from a UME6/ume6 heterozygote exhibited reduced germination efficiency compared with their wild-type sister spores. Analysis of ume6 spore colonies that did germinate revealed that the majority of cells in microcolonies following the first few cell divisions were inviable. As the colony developed, the viability percentage increased and achieved wild-type levels within approximately six cell divisions, indicating that the requirement for Ume6p in cell viability is transient. This function is specific for germinating spores as Ume6p has no or only a modest impact on the return to the growth ability of cells arrested at other points in the cell cycle. These results define a new role for Ume6p in spore germination and the first few subsequent mitotic cell divisions.