Editor: David Goldfarb
SIR2 and other genes are abundantly expressed in long-lived natural segregants for replicative aging of the budding yeast Saccharomyces cerevisiae
Article first published online: 1 MAR 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Yeast Research
Volume 11, Issue 4, pages 345–355, June 2011
How to Cite
Guo, Z., Adomas, A. B., Jackson, E. D., Qin, H. and Townsend, J. P. (2011), SIR2 and other genes are abundantly expressed in long-lived natural segregants for replicative aging of the budding yeast Saccharomyces cerevisiae. FEMS Yeast Research, 11: 345–355. doi: 10.1111/j.1567-1364.2011.00723.x
- Issue published online: 9 MAY 2011
- Article first published online: 1 MAR 2011
- Accepted manuscript online: 9 FEB 2011 08:32AM EST
- Received 26 February 2010; revised 23 January 2011; accepted 24 January 2011., Final version published online 1 March 2011.
- gene expression;
- life span;
We investigated the mechanism underlying the natural variation in longevity within natural populations using the model budding yeast, Saccharomyces cerevisiae. We analyzed whole-genome gene expression in four progeny of a natural S. cerevisiae strain that display differential replicative aging. Genes with different expression levels in short- and long-lived strains were classified disproportionately into metabolism, transport, development, transcription or cell cycle, and organelle organization (mitochondrial, chromosomal, and cytoskeletal). With several independent validating experiments, we detected 15 genes with consistent differential expression levels between the long- and the short-lived progeny. Among those 15, SIR2, HSP30, and TIM17 were upregulated in long-lived strains, which is consistent with the known effects of gene silencing, stress response, and mitochondrial function on aging. The link between SIR2 and yeast natural life span variation offers some intriguing ties to the allelic association of the human homolog SIRT1 to visceral obesity and metabolic response to lifestyle intervention.