Research Article

Alcohol-based quorum sensing plays a role in adhesion and sliding motility of the yeast Debaryomyces hansenii

  1. Klaus Gori1,
  2. Peter B. Knudsen2,
  3. Kristian F. Nielsen2,
  4. Nils Arneborg1,*,
  5. Lene Jespersen1

Article first published online: 20 OCT 2011

DOI: 10.1111/j.1567-1364.2011.00755.x

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 11, Issue 8, pages 643–652, December 2011

Additional Information

How to Cite

Gori, K., Knudsen, P. B., Nielsen, K. F., Arneborg, N. and Jespersen, L. (2011), Alcohol-based quorum sensing plays a role in adhesion and sliding motility of the yeast Debaryomyces hansenii. FEMS Yeast Research, 11: 643–652. doi: 10.1111/j.1567-1364.2011.00755.x

Author Information

  1. 1

    Department of Food Science, Food Microbiology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark

  2. 2

    Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Lyngby, Denmark

*Correspondence: Nils Arneborg, Department of Food Science, Food Microbiology, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Tel.: +45 35333266; fax:+45 35333215; e-mail: na@life.ku.dk

Publication History

  1. Issue published online: 24 OCT 2011
  2. Article first published online: 20 OCT 2011
  3. Accepted manuscript online: 19 SEP 2011 09:07AM EST
  4. Manuscript Accepted: 5 SEP 2011
  5. Manuscript Revised: 4 SEP 2011
  6. Manuscript Received: 12 MAY 2011

Funded by

  • The Danish Dairy Research Foundation (Danish Dairy Board)
  • The Danish Ministry of Food, Agriculture and Fisheries

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Keywords:

  • Debaryomyces hansenii;
  • alcohol-based quorum sensing;
  • adhesion;
  • biofilm formation;
  • sliding motility

Abstract

The yeast Debaryomyces hansenii was investigated for its production of alcohol-based quorum sensing (QS) molecules including the aromatic alcohols phenylethanol, tyrosol, tryptophol and the aliphatic alcohol farnesol. Debaryomyces hansenii produced phenylethanol and tyrosol, which were primarily detected from the end of exponential phase indicating that they are potential QS molecules in D. hansenii as previously shown for other yeast species. Yields of phenylethanol and tyrosol produced by D. hansenii were, however, lower than those produced by Candida albicans and Saccharomyces cerevisiae and varied with growth conditions such as the availability of aromatic amino acids, ammonium sulphate, NaCl, pH and temperature. Tryptophol was only produced in the presence of tryptophane, whereas farnesol in general was not detectable. Especially, the type strain of D. hansenii (CBS767) had good adhesion and sliding motility abilities, which seemed to be related to a higher hydrophobicity of the cell surface of D. hansenii (CBS767) rather than the ability to form pseudomycelium. Addition of phenylethanol, tyrosol, tryptophol and farnesol was found to influence both adhesion and sliding motility of D. hansenii.

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