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Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae

Authors

  • Jin Hou,

    1. Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
    2. State Key Laboratory of Microbial Technology, Shandong University, Jinan, China
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  • Keith E.J. Tyo,

    1. Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
    2. Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
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  • Zihe Liu,

    1. Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
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  • Dina Petranovic,

    1. Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
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  • Jens Nielsen

    Corresponding author
    • Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
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Correspondence: Jens Nielsen, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Göteborg, Sweden. Tel.: +46 31 772 3804; fax: +46 31 772 3801;

e-mail: nielsenj@chalmers.se

Abstract

The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels and chemicals, and it is also provides a platform for the production of many heterologous proteins of medical or industrial interest. Therefore, many studies have focused on metabolic engineering S. cerevisiae to improve the recombinant protein production, and with the development of systems biology, it is interesting to see how this approach can be applied both to gain further insight into protein production and secretion and to further engineer the cell for improved production of valuable proteins. In this review, the protein post-translational modification such as folding, trafficking, and secretion, steps that are traditionally studied in isolation will here be described in the context of the whole system of protein secretion. Furthermore, examples of engineering secretion pathways, high-throughput screening and systems biology applications of studying protein production and secretion are also given to show how the protein production can be improved by different approaches. The objective of the review is to describe individual biological processes in the context of the larger, complex protein synthesis network.

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