In vitro cultivation of parthenogenesis-inducing Wolbachia in an Aedes albopictus cell line


*Correspondence: Kazuki Miura, National Agricultural Research Center for Western Region, Fukuyama 721-8514, Japan. E-mail:


Parthenogenesis-inducing (PI) Wolbachia, infecting the parasitoid wasp Trichogramma kaykai Pinto and Stouthamer (Hymenoptera: Trichogrammatidae), were successfully maintained and cultivated in a mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae), cell line, NIAS-AeAl-2. A parthenogenesis-inducing (PI) Wolbachia strains with wasp ovaries were introduced into flasks with cultures of the cell line. The PI Wolbachia proliferated in NIAS-AeAl-2 as confirmed using a quantitative polymerase chain reaction method. Our final goal is to induce parthenogenesis in other hymenopteran species of commercial interest through the artificial transfer of PI Wolbachia. As a step towards this goal, we microinjected pupae of Trichogramma with PI Wolbachia culture in NIAS-AeAl-2 cells and PI Wolbachia was detected from recipient by PCR with Wolbachia specific primers (wsp gene).