Biotechnology Laboratory, Department of Civil Engineering, Aalborg University, Sohngaardholmsvej 57, 9000 Aalborg, Denmark.
Spectrochromatography of photosynthetic pigments as a fingerprinting technique for microbial phototrophs
Article first published online: 17 JAN 2006
FEMS Microbiology Ecology
Volume 20, Issue 2, pages 69–77, June 1996
How to Cite
Frigaard, N.-U., Larsen, K. L. and Cox, R. P. (1996), Spectrochromatography of photosynthetic pigments as a fingerprinting technique for microbial phototrophs. FEMS Microbiology Ecology, 20: 69–77. doi: 10.1111/j.1574-6941.1996.tb00306.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 11 August 1995, Revised 4 January 1996, Accepted 8 January 1996)
- High-performance liquid chromatography;
- Diode-array spectrophotometry;
- Microbial phototroph
Abstract: The combination of chromatographic separation using reverse-phase high-performance liquid chromatography and continuous monitoring of the column eluate using a diode-array spectrophotometer allowed qualitative and quantitative pigment profiling of extracts of photosynthetic material in a single run. Carotenoids and the spectrally distinct types of chlorophyll and bacteriochlorophyll can be unambiguously identified even when imperfectly separated on the column. The resulting spectrochromatogram is a fingerprint useful for the rapid characterization of pure cultures or mixed populations. We have developed software to allow recording, manipulation, and presentation of the resulting spectrochromatograms and present results from photosynthetic microbes in pure and mixed cultures. We describe a number of approaches to the presentation of the resulting data in a readily comprehensible form.