Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA

Authors

  • Sergey R. Konstantinov,

    1. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, Netherlands
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  • Wei-Yun Zhu,

    1. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, Netherlands
    2. Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, Netherlands
    3. College of Animal Science and Technology, Nanjing Agricultural University, 210095 Nanjing, PR China
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  • Barbara A. Williams,

    1. Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, Netherlands
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  • Seerp Tamminga,

    1. Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, Netherlands
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  • Willem M. de Vos,

    1. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, Netherlands
    2. Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN Wageningen, Netherlands
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  • Antoon D.L. Akkermans

    Corresponding author
    1. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, Netherlands
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*Corresponding author. Tel.: +31 (317) 48 34 86; Fax: +31 (317) 48 38 29. antoon.akkermans@algemeen.micr.wag-ur.nl

Abstract

The effect of fermentable carbohydrates (sugar beet pulp and fructooligosaccharides) on the faecal bacterial communities of weaning piglets was analysed using 16S rDNA-based approaches. Amplicons of the V6–V8 variable regions of bacterial 16S rDNA were analysed by denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Differences in piglet faecal bacterial community structure were determined based on the Dice coefficients for pairwise comparison of the DGGE fingerprints and revealed significant changes in the faecal microbiota immediately after weaning. Piglets fed with fermentable carbohydrates showed a higher bacterial diversity and a more rapid stabilisation of the bacterial community compared with that of the animals fed with the control diet. Thirteen dominant DGGE bands were matched with sequences that showed 91–97% similarity to those derived from the Clostridium coccoides group and the Clostridium leptum subgroup. Amplicons related to Ruminococcus-like species were found in all DGGE fingerprints derived from pigs on the diet containing sugar beet pulp and fructooligosaccharides, but not in pigs on the control diet. These results indicate that these bacteria may play a role in the utilisation of dietary fibres.

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