Editor: Julian Marchesi
Effects of naphthalene on microbial community composition in the Delaware estuary
Article first published online: 24 JAN 2006
FEMS Microbiology Ecology
Volume 56, Issue 1, pages 55–63, April 2006
How to Cite
Castle, D. M., Montgomery, M. T. and Kirchman, D. L. (2006), Effects of naphthalene on microbial community composition in the Delaware estuary. FEMS Microbiology Ecology, 56: 55–63. doi: 10.1111/j.1574-6941.2006.00062.x
Present address: Dawn M. Castle, University of Tennessee at Chattanooga, Biological and Environmental Sciences, 615 McCallie Avenue, Chattanooga, TN 37403, USA.
- Issue published online: 8 FEB 2006
- Article first published online: 24 JAN 2006
- Received 13 April 2005; revised 12 October 2005; accepted 13 October 2005.
- polycyclic aromatic hydrocarbons;
- denaturing gradient gel electrophoresis;
- fluorescence in situ hybridization
The effects of naphthalene on microbial communities in the bottom boundary layer of the Delaware Bay estuary were investigated in microcosms using denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) with oligonucleotide probes. Three days after the addition of naphthalene, rates of bacterial production and naphthalene mineralization were higher than in no-addition controls and than in cases where glucose was added. Analyses using both DGGE and FISH indicated that the bacterial community changed in response to the addition of naphthalene. FISH data indicated that a few major phylogenetic groups increased in response to the glucose addition and especially to the naphthalene addition. DGGE also demonstrated differences in community composition among treatments, with four phylotypes being unique to naphthalene-amended treatments and three of these having 16S rRNA genes similar to known hydrocarbon degraders. The bacterial community in the naphthalene-amended treatment was distinct from the communities in the glucose-amended treatment and in the no-addition control. These data suggest that polycyclic aromatic hydrocarbons may have large effects on microbial community structure in estuaries and probably on microbially mediated biogeochemical processes.